Cytocompatibility between novel nerve conduit composite materials and bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.16.009

Abstract

Abstract:

BACKGROUND: Ideal nerve repair materials are characterized as good biocompatibility, biodegradability, plasticity, and certain mechanical strength.
OBJECTIVE: To evaluate the cytocompatibility of rat bone marrow mesenchymal stem cells with (glycolic acid-L-lysine-lactic acid)/poly lactic acid/β-tricalcium phosphate/nerve growth factor composites.
METHODS: Passage 3 bone marrow mesenchymal stem cells which were isolated from Sprague-Dawley rats were co-cultured with (glycolic acid-L-lysine-lactic acid)/poly lactic acid/β-tricalcium phosphate/nerve growth factor composite materials or their extracts, serving as experimental control. Another bone marrow mesenchymal stem cells cultured in the culture medium containing 10% fetal bovine serum served as controls. Cell growth, survival and apoptosis on the composite materials were observed.
RESULTS AND CONCLUSION: Results from 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that absorbance values in the experimental group were higher than those in the control group after co-culture for days 5 and 7 (P < 0.05). Annexin V-FITC/PI double staining results showed that the apoptosis rate of the experimental group was significantly lower than that of the control group (P < 0.05). Under a scanning electron microscope, bone marrow mesenchymal stem cells grew well on the surface of (glycolic acid-L-lysine-lactic acid)/poly lactic acid/β-tricalcium phosphate/nerve growth factor composite materials. Many protrusions ran out from the cells, and they were woven into a mesh, which was typical performance of neuron-like cells. These findings indicate that (glycolic acid-L-lysine-lactic acid)/poly lactic acid/β-tricalcium phosphate/nerve growth factor composite materials have good cytocompatibility with bone marrow mesenchymal stem cells, which can be regarded as an excellent carrier for artificial nerve construction.

Key words: biomaterials, material biocompatibility, peripheral nerve injury, (glycolic acid-L-lysine-lactic acid)/poly lactic acid/beta-tricalcium phosphate/nerve growth factor, bone marrow mesenchymal stem cells, apoptosis, artificial nerve, nerve tissue engineering, other grants-supported paper

CLC Number:

R318

Hu Hui, Zhang Wei-cai, Huang Ji-feng, Yan Qiong-jiao, Yin Yi-xia, Li Shi-pu. Cytocompatibility between novel nerve conduit composite materials and bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2913-2920.

Figures/Tables 6

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